Homoeologous gene silencing in hexaploid wheat

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RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat.

Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gain- or loss-of-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum aestivum), where most of the genes a...

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Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat.

Bread wheat (Triticum aestivum) is a hexaploid species with A, B, and D ancestral genomes. Most bread wheat genes are present in the genome as triplicated homoeologous genes (homoeologs) derived from the ancestral species. Here, we report that both genetic and epigenetic alterations have occurred in the homoeologs of a wheat class E MADS box gene. Two class E genes are identified in wheat, whea...

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Gene expression profiles of hybrid necrosis in synthetic hexaploid wheat

Some abnormal growth phenotypes including hybrid necrosis are often observed in F1 hybrids (genome constitution, ABD, 2n=21) and synthetic hexaploid wheat lines (AABBDD, 2n=42) which were artificially produced by crossing between tetraploid wheat (AABB, 2n=28) and Ae. tauschii (DD, 2n=14). The hybrid necrosis phenotypes are generally divided into type I and type II necrosis. In the hybrid plant...

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1 Non - Additive Expression of Homoeologous Genes is Established Upon Polyploidization in 2 Hexaploid Wheat 3 4 5

44 Effects of polyploidy in allohexaploid wheat (Triticum aestivum L.) have primarily been ascribed 45 to increases in coding sequence variation and potential to acquire new gene functions through 46 mutation of redundant loci. However, regulatory variation that arises through new promoter and 47 transcription factor combinations or epigenetic events may also contribute to the effects of 48 pol...

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Genome Analysis RNA Interference-Based Gene Silencing as an Efficient Tool for Functional Genomics in Hexaploid Bread Wheat

Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gainor lossof-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum aestivum), where most of the genes are ...

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ژورنال

عنوان ژورنال: The Plant Journal

سال: 2006

ISSN: 0960-7412,1365-313X

DOI: 10.1111/j.1365-313x.2006.02841.x